This study was carried out in a randomized block design in a 2×2 factorial arrangement with the aim of evaluating the short-term changes in the plasma and milk fatty acid (FA) profiles of Holstein x Gyr cows (444±84 kg; 75±31 days in milk; 15.4±4.8 kg day-1 of milk) fed 600 g kg-1 DM chopped elephant grass-based diets supplemented with 45 g kg-1 DM sunflower oil (SO) for 21 days. Two types of SO were evaluated: high oleic/low linoleic acid (HO) and medium oleic/medium linoleic acid (MO). The concentrates containing SO were supplied separately from forage twice a day (TAD) or as part of a total mixed ration (TMR). Temporal changes were analyzed by mixed models (P < 0.05) using six, four and seven repeated measures over time for FA intake and the plasma and milk FA compositions, respectively. There was higher oleic acid intake in HO SO-fed...

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