Blueberry consumption has increased in recent years, partially due to the health benefits attributed to their phenolic content. This study aimed to optimize and validate a high-performance liquid chromatography/quadrupole-time of flight mass spectrometry (HPLC/QTOF-MS) methodology for the determination of free (i.e., solvent extractable) and bound (i.e., covalently bound to the plant cellular structures) phenolics in northern highbush blueberries. A total of 31 phenolics, including anthocyanins, flavonols, flavan-3-ols, and phenolic acids, were targeted in the positive electrospray ionization mde [ESI (+)], including MS/MS confirmation of their product ions. Free phenolics determination was validated in terms of sensitivity, reproducibility, linearity, and accuracy, and 21 free phenolic compounds were found above their limits of detection among six commercial blueberry cultivars, which were quantified with correction for the matrix effect. "Elliott" presented the highest free phenolic and anthocyanin contents (3640 and 3430 mg kg-1 fresh weight, respectively). Malvidin-3-O-galactoside was the...

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