Aims. To compare the heat stability of two globally prevalent human norovirus (HuNoV) strains (GII. 2 [P16] and GII. 4 [P16]) and a commonly used HuNoV surrogate, tulane virus (TV). Methods and Results. With the use of a newly developed zebrafish larvae platform, we measured the change of infectivity of HuNoV GII. 2 [P16] and GII. 4 [P16] toward mild heat treatment at 55 °C for 5 min. TV was tested with the same experimental design. As a result, the virus infectivity measurement clearly indicated the higher heat resistance of HuNoV GII. 2 [P16] (no reduction) than GII. 4 [P16] (>0.8-log TCID50 ml-1 reduction) and TV (2.5-log TCID50 ml-1 reduction). Further exploration revealed higher virus structural stability of HuNoV GII. 2 than GII. 4 strains by the use of different clinical samples with different evaluation methods. Conclusion. The inactivation data generated from the surrogate virus TV...

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