A method of reducing stress level in animals such as pigs may comprise: sealable enclosing the animals in at least one chamber; exposing the animals to a gas mixture comprising oxygen and at least one type of physiologically inert gas; gradually decreasing the oxygen concentration from a maximum level, to a minimum level; gradually increasing the at least one physiologically inert gas concentration from a minimum level to a maximum level; wherein the minimum level of the oxygen concentration is higher than the anaesthetic-causing concentration for the animals.

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