The present invention aims to provide a thermo-acidophilic Bacilli inhibitor, a method of preventing contamination by thermo-acidophilic Bacilli, and a beverage, which utilize as an active ingredient a substance having the effect of inhibiting the growth of thermo-acidophilic Bacilli. The present invention relates to a thermo-acidophilic Bacilli inhibitor containing xanthohumol as an active ingredient, a method of preventing contamination by thermo-acidophilic Bacilli using the same, and a beverage containing the same.

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